Methods for the isolation and analysis of plasmodial mutants in Physarum polycephalum
نویسندگان
چکیده
منابع مشابه
Complementation of Amoebal-Plasmodial Transition Mutants in PHYSARUM POLYCEPHALUM.
Amoebae of the Myxomycete Physarum polycephalum differentiate to yield plasmodia in two ways: in crossing, haploid amoebae of appropriate genotypes fuse to form diploid plasmodia; in selfing, plasmodia form without amoebal fusion or increase in ploidy. Amoebae carrying the mating-type allele matAh (formerly mt(h)) self efficiently, but occasionally give rise to mutants that self at very low fre...
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Amoebae of Physarum polycephalum carrying the mth mating-type allele may differentiate into plasmodia in the absence of mating. Such plasmodia are haploid and, upon sporulation, produce mainly inviable spores. We have asked whether the viable spores arise from meiotic or mitotic divisions. Using a microfluorometric measurement of the deoxyribonucleic acid content of individual nuclei, we found ...
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ژورنال
عنوان ژورنال: Genetical Research
سال: 1974
ISSN: 0016-6723,1469-5073
DOI: 10.1017/s0016672300015202